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luminescent camp probe glosensor-22f  (Promega)

 
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    Promega luminescent camp probe glosensor-22f
    DOM reduces the PT-mediated effects on cAMP signaling. Cells were treated with DOM at indicated concentrations and PT (100 ng/mL) for 5 h at 37 °C or cells were left untreated (con). Inducing medium with luciferase substrate for the <t>luminescent</t> cAMP biosensor was added. Baseline was measured for 15 min, then cells were spiked with forskolin (FSK, activator of adenylate cyclase) and octreotide acetate (Oct, activator of SSTR2). Luminescence was recorded for 60 min. ( a ) cAMP kinetic curves of one representative experiment are shown. Values are given as mean ± SD, n ≥ 3 from one experiment. ( b ) Bar graph shows baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of samples treated with PT only, mean ± SEM, n ≥ 6. ( c ) As the control, cells were treated with FSK or PT plus FSK without Oct to detect maximal cAMP response. Values for the control samples and samples treated with PT, FSK, and Oct are identical to values in ( b ). Significance was tested by one-way ANOVA analysis and Dunnett’s multiple comparisons test, and values refer to samples treated with PT only (left graph in ( b , c ), white bar) or con (right graph in ( b ), white bar), (**** p < 0.0001, *** p < 0.001, * p < 0.05, ns = not significant p > 0.05).
    Luminescent Camp Probe Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luminescence+camp+probe+glosensor-22f/luminescent+camp+probe+glosensor+22f/pmc10467066-215-22-26
    Average 90 stars, based on 1 article reviews
    luminescent camp probe glosensor-22f - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Domperidone Inhibits Clostridium botulinum C2 Toxin and Bordetella pertussis Toxin"

    Article Title: Domperidone Inhibits Clostridium botulinum C2 Toxin and Bordetella pertussis Toxin

    Journal: Toxins

    doi: 10.3390/toxins15070412

    DOM reduces the PT-mediated effects on cAMP signaling. Cells were treated with DOM at indicated concentrations and PT (100 ng/mL) for 5 h at 37 °C or cells were left untreated (con). Inducing medium with luciferase substrate for the luminescent cAMP biosensor was added. Baseline was measured for 15 min, then cells were spiked with forskolin (FSK, activator of adenylate cyclase) and octreotide acetate (Oct, activator of SSTR2). Luminescence was recorded for 60 min. ( a ) cAMP kinetic curves of one representative experiment are shown. Values are given as mean ± SD, n ≥ 3 from one experiment. ( b ) Bar graph shows baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of samples treated with PT only, mean ± SEM, n ≥ 6. ( c ) As the control, cells were treated with FSK or PT plus FSK without Oct to detect maximal cAMP response. Values for the control samples and samples treated with PT, FSK, and Oct are identical to values in ( b ). Significance was tested by one-way ANOVA analysis and Dunnett’s multiple comparisons test, and values refer to samples treated with PT only (left graph in ( b , c ), white bar) or con (right graph in ( b ), white bar), (**** p < 0.0001, *** p < 0.001, * p < 0.05, ns = not significant p > 0.05).
    Figure Legend Snippet: DOM reduces the PT-mediated effects on cAMP signaling. Cells were treated with DOM at indicated concentrations and PT (100 ng/mL) for 5 h at 37 °C or cells were left untreated (con). Inducing medium with luciferase substrate for the luminescent cAMP biosensor was added. Baseline was measured for 15 min, then cells were spiked with forskolin (FSK, activator of adenylate cyclase) and octreotide acetate (Oct, activator of SSTR2). Luminescence was recorded for 60 min. ( a ) cAMP kinetic curves of one representative experiment are shown. Values are given as mean ± SD, n ≥ 3 from one experiment. ( b ) Bar graph shows baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of samples treated with PT only, mean ± SEM, n ≥ 6. ( c ) As the control, cells were treated with FSK or PT plus FSK without Oct to detect maximal cAMP response. Values for the control samples and samples treated with PT, FSK, and Oct are identical to values in ( b ). Significance was tested by one-way ANOVA analysis and Dunnett’s multiple comparisons test, and values refer to samples treated with PT only (left graph in ( b , c ), white bar) or con (right graph in ( b ), white bar), (**** p < 0.0001, *** p < 0.001, * p < 0.05, ns = not significant p > 0.05).

    Techniques Used: Luciferase, Control

    Related Articles

    Expressing:

    Article Title: Human Peptides α-Defensin-1 and -5 Inhibit Pertussis Toxin
    Article Snippet: .. HEK293 cells ectopically expressing Gαi-coupled somatostatin receptor 2 (SSTR2) GPCR as well as a luminescence cAMP probe (GloSensor-22F, Promega) were seeded in 96-well plates with white walls and a translucent bottom (View-Plate 96, Perkin Elmer). .. PT (100 ng/mL) or matched buffer SolC (50% glycerol, 50 mM Tris, 10 mM glycine, 0.5 M NaCl, pH 7.5) and defensins (12 μM) or H 2 O as a control were pre-incubated in FCS-free medium for 15 minutes and then added to the cells for 5 h. Subsequently, medium was removed and 45 μL inducing medium (comprised of 2% GloSensor reagent (Promega), 400 μM of the phosphodiesterase inhibitor IBMX (Sigma) in DMEM/F12 medium and CO 2 -independent medium (4v of DMEM/F12 per 5v CO 2 -independent medium), supplemented with 0.1% (w/v) bovine serum albumin) was added and equilibrated for 45 min at room temperature in the dark.

    Article Title: Human Peptides α-Defensin-1 and -5 Inhibit Pertussis Toxin
    Article Snippet: Chinese hamster ovary cells strain K1 (CHO-K1, DSMZ, Braunschweig, Germany) were cultivated in DMEM and HAM’s F12 (1:1) supplemented with 5% heat-inactivated fetal calf serum (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), 1 mM sodium pyruvate and penicillin–streptomycin (1:100) (Thermo Fisher Scientific, Waltham, MA, USA). .. HEK293 cells (HEK-Gs/SSTR2_HA), ectopically expressing Gαi-coupled somatostatin receptor 2 (SSTR2) GPCR as well as a luminescence cAMP probe (GloSensor-22F, Promega, Mannheim, Germany) [ ], were cultivated in DMEM/F12 (containing glutamine and sodium pyruvate) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen) and penicillin–streptomycin (1:100) (Thermo Fisher Scientific). ..



    Similar Products

    luminescent camp probe glosensor-22f  (Promega)
    90
    Promega luminescent camp probe glosensor-22f
    DOM reduces the PT-mediated effects on cAMP signaling. Cells were treated with DOM at indicated concentrations and PT (100 ng/mL) for 5 h at 37 °C or cells were left untreated (con). Inducing medium with luciferase substrate for the <t>luminescent</t> cAMP biosensor was added. Baseline was measured for 15 min, then cells were spiked with forskolin (FSK, activator of adenylate cyclase) and octreotide acetate (Oct, activator of SSTR2). Luminescence was recorded for 60 min. ( a ) cAMP kinetic curves of one representative experiment are shown. Values are given as mean ± SD, n ≥ 3 from one experiment. ( b ) Bar graph shows baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of samples treated with PT only, mean ± SEM, n ≥ 6. ( c ) As the control, cells were treated with FSK or PT plus FSK without Oct to detect maximal cAMP response. Values for the control samples and samples treated with PT, FSK, and Oct are identical to values in ( b ). Significance was tested by one-way ANOVA analysis and Dunnett’s multiple comparisons test, and values refer to samples treated with PT only (left graph in ( b , c ), white bar) or con (right graph in ( b ), white bar), (**** p < 0.0001, *** p < 0.001, * p < 0.05, ns = not significant p > 0.05).
    Luminescent Camp Probe Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luminescence+camp+probe+glosensor-22f/luminescent+camp+probe+glosensor+22f/pmc10467066-215-22-26
    Average 90 stars, based on 1 article reviews
    luminescent camp probe glosensor-22f - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    luminescence camp probe glosensor-22f  (Promega)
    90
    Promega luminescence camp probe glosensor-22f
    Human α-defensin-1 and -5 inhibit PT-mediated effects on cAMP signaling. PT (100 ng/mL) was pre-incubated with 12 µM α-defensin-1, α-defensin-5 or ß-defensin-1, or with the respective amount of solvent (H 2 O) for 15 min at room temperature and then added to iGIST sensor cells for 5 h at 37°C. For further control, cells were treated only with the solvent of PT. Then, inducing medium containing the luciferase substrate for the luminescent biosensor for cAMP was added. After 15 minutes of baseline measurement, cells were spiked with forskolin to activate adenylate cyclase and octreotide acetate to activate Gαi-coupled SSTR2 GPCR. <t>Luminescence</t> was recorded for further 60 minutes. ( a ) cAMP kinetic curves from one representative experiment are shown as mean ± SD (n = 3 from one experiment), con = cells treated only with forskolin (FSK) plus octreotide (Oct). ( b ) Bar graphs show baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of PT-only-treated samples, mean ± SEM (n = at least nine from at least three independent experiments). Values for control samples are identical in both graphs. Results are shown in two separate graphs for better clarity. Significance was tested using one-way ANOVA followed by Dunnett’s multiple comparison test and refers to samples treated with PT only (left graph) or untreated controls (right graph) (** p ≤ 0.01,**** p ≤ 0.0001, ns not significant). ( c ) For control, iGIST sensor cells were treated only with FSK or with PT plus FSK in the absence of Oct to measure the maximal cAMP response in this assay. Values for control samples and samples treated with PT plus FSK and Oct are identical to values in ( a ).
    Luminescence Camp Probe Glosensor 22f, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/luminescence+camp+probe+glosensor-22f/luminescence+camp+probe+glosensor+22f/pmc08310310-122-15-19
    Average 90 stars, based on 1 article reviews
    luminescence camp probe glosensor-22f - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    DOM reduces the PT-mediated effects on cAMP signaling. Cells were treated with DOM at indicated concentrations and PT (100 ng/mL) for 5 h at 37 °C or cells were left untreated (con). Inducing medium with luciferase substrate for the luminescent cAMP biosensor was added. Baseline was measured for 15 min, then cells were spiked with forskolin (FSK, activator of adenylate cyclase) and octreotide acetate (Oct, activator of SSTR2). Luminescence was recorded for 60 min. ( a ) cAMP kinetic curves of one representative experiment are shown. Values are given as mean ± SD, n ≥ 3 from one experiment. ( b ) Bar graph shows baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of samples treated with PT only, mean ± SEM, n ≥ 6. ( c ) As the control, cells were treated with FSK or PT plus FSK without Oct to detect maximal cAMP response. Values for the control samples and samples treated with PT, FSK, and Oct are identical to values in ( b ). Significance was tested by one-way ANOVA analysis and Dunnett’s multiple comparisons test, and values refer to samples treated with PT only (left graph in ( b , c ), white bar) or con (right graph in ( b ), white bar), (**** p < 0.0001, *** p < 0.001, * p < 0.05, ns = not significant p > 0.05).

    Journal: Toxins

    Article Title: Domperidone Inhibits Clostridium botulinum C2 Toxin and Bordetella pertussis Toxin

    doi: 10.3390/toxins15070412

    Figure Lengend Snippet: DOM reduces the PT-mediated effects on cAMP signaling. Cells were treated with DOM at indicated concentrations and PT (100 ng/mL) for 5 h at 37 °C or cells were left untreated (con). Inducing medium with luciferase substrate for the luminescent cAMP biosensor was added. Baseline was measured for 15 min, then cells were spiked with forskolin (FSK, activator of adenylate cyclase) and octreotide acetate (Oct, activator of SSTR2). Luminescence was recorded for 60 min. ( a ) cAMP kinetic curves of one representative experiment are shown. Values are given as mean ± SD, n ≥ 3 from one experiment. ( b ) Bar graph shows baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of samples treated with PT only, mean ± SEM, n ≥ 6. ( c ) As the control, cells were treated with FSK or PT plus FSK without Oct to detect maximal cAMP response. Values for the control samples and samples treated with PT, FSK, and Oct are identical to values in ( b ). Significance was tested by one-way ANOVA analysis and Dunnett’s multiple comparisons test, and values refer to samples treated with PT only (left graph in ( b , c ), white bar) or con (right graph in ( b ), white bar), (**** p < 0.0001, *** p < 0.001, * p < 0.05, ns = not significant p > 0.05).

    Article Snippet: The iGIST bioassay [ ] is based on HEK293 cells that ectopically express the Gαi-coupled somatostatin receptor 2 (SSTR2) GPCR and a luminescent cAMP probe (GloSensor-22F, Promega, Madison, WI, USA).

    Techniques: Luciferase, Control

    Human α-defensin-1 and -5 inhibit PT-mediated effects on cAMP signaling. PT (100 ng/mL) was pre-incubated with 12 µM α-defensin-1, α-defensin-5 or ß-defensin-1, or with the respective amount of solvent (H 2 O) for 15 min at room temperature and then added to iGIST sensor cells for 5 h at 37°C. For further control, cells were treated only with the solvent of PT. Then, inducing medium containing the luciferase substrate for the luminescent biosensor for cAMP was added. After 15 minutes of baseline measurement, cells were spiked with forskolin to activate adenylate cyclase and octreotide acetate to activate Gαi-coupled SSTR2 GPCR. Luminescence was recorded for further 60 minutes. ( a ) cAMP kinetic curves from one representative experiment are shown as mean ± SD (n = 3 from one experiment), con = cells treated only with forskolin (FSK) plus octreotide (Oct). ( b ) Bar graphs show baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of PT-only-treated samples, mean ± SEM (n = at least nine from at least three independent experiments). Values for control samples are identical in both graphs. Results are shown in two separate graphs for better clarity. Significance was tested using one-way ANOVA followed by Dunnett’s multiple comparison test and refers to samples treated with PT only (left graph) or untreated controls (right graph) (** p ≤ 0.01,**** p ≤ 0.0001, ns not significant). ( c ) For control, iGIST sensor cells were treated only with FSK or with PT plus FSK in the absence of Oct to measure the maximal cAMP response in this assay. Values for control samples and samples treated with PT plus FSK and Oct are identical to values in ( a ).

    Journal: Toxins

    Article Title: Human Peptides α-Defensin-1 and -5 Inhibit Pertussis Toxin

    doi: 10.3390/toxins13070480

    Figure Lengend Snippet: Human α-defensin-1 and -5 inhibit PT-mediated effects on cAMP signaling. PT (100 ng/mL) was pre-incubated with 12 µM α-defensin-1, α-defensin-5 or ß-defensin-1, or with the respective amount of solvent (H 2 O) for 15 min at room temperature and then added to iGIST sensor cells for 5 h at 37°C. For further control, cells were treated only with the solvent of PT. Then, inducing medium containing the luciferase substrate for the luminescent biosensor for cAMP was added. After 15 minutes of baseline measurement, cells were spiked with forskolin to activate adenylate cyclase and octreotide acetate to activate Gαi-coupled SSTR2 GPCR. Luminescence was recorded for further 60 minutes. ( a ) cAMP kinetic curves from one representative experiment are shown as mean ± SD (n = 3 from one experiment), con = cells treated only with forskolin (FSK) plus octreotide (Oct). ( b ) Bar graphs show baseline-subtracted area under the curve (AUC) from at least three independent experiments. Values are given as percent of PT-only-treated samples, mean ± SEM (n = at least nine from at least three independent experiments). Values for control samples are identical in both graphs. Results are shown in two separate graphs for better clarity. Significance was tested using one-way ANOVA followed by Dunnett’s multiple comparison test and refers to samples treated with PT only (left graph) or untreated controls (right graph) (** p ≤ 0.01,**** p ≤ 0.0001, ns not significant). ( c ) For control, iGIST sensor cells were treated only with FSK or with PT plus FSK in the absence of Oct to measure the maximal cAMP response in this assay. Values for control samples and samples treated with PT plus FSK and Oct are identical to values in ( a ).

    Article Snippet: HEK293 cells (HEK-Gs/SSTR2_HA), ectopically expressing Gαi-coupled somatostatin receptor 2 (SSTR2) GPCR as well as a luminescence cAMP probe (GloSensor-22F, Promega, Mannheim, Germany) [ ], were cultivated in DMEM/F12 (containing glutamine and sodium pyruvate) supplemented with 10% heat-inactivated fetal calf serum (Invitrogen) and penicillin–streptomycin (1:100) (Thermo Fisher Scientific).

    Techniques: Incubation, Solvent, Control, Luciferase, Comparison